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产品概述
名称 | RAW 264.7 (小鼠单核巨噬细胞白血病细胞) (STR鉴定正确) |
别称 | RAW264; RAW2647; RAW264.7; RAW-264.7; Raw 264.7; Raw264.7 |
背景描述 | RAW 264.7细胞源自Abelson鼠科白血病病毒诱导的肿瘤;sIg-、Ia-抗原、Thy-1.2表面抗原阴性。RAW 264.7细胞不分泌可检测到的病毒颗粒,XC斑点形成试验阴性。RAW 264.7细胞可以胞饮中性红并吞噬乳胶颗粒与酵母聚糖,并且可以抗体依赖性地分解绵羊红血球与肿瘤靶细胞。LPS或PPD处理2天可诱导RAW 264.7细胞分解红血球,但对肿瘤靶细胞无作用。 |
种属 | 小鼠 |
年龄(性别) | 雄性,成年 |
组织来源 | Abelson鼠科白血病病毒诱导的肿瘤;单核细胞;巨噬细胞 |
生长特性 | 贴壁细胞,不用胰酶消化 |
细胞形态 | 不规则形 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 白血病细胞 |
生物安全等级 | 2 |
生长培养基 | DMEM+10% FBS+1% P/S |
推荐传代比例 | 1:6~1:8 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~12-30小时 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
受体表达情况 | complement (C3) |
抗原表达情况 | H-2d |
基因表达情况 | lysozyme, H-2d; Tested and found negative for ectromelia virus (mousepox). |
注意事项 | 1、该细胞不建议使用胰酶消化,胰酶会刺激分化; 2、 超过3天不传代细胞容易分化; 3、 培养时,存在少量分化细胞,属于正常现象。 |
保藏机构 | ATCC; TIB-71 ECACC; 91062702 |
STR鉴定
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STR位点信息
18-3 18 6-7 12 5-5 14 X-1 24 1-2 17 7-1 25.2 8-1 13 1-1 15,16 19-2 14 15-3 22.3 12-1 16 6-4 18 4-2 22.3 3-2 14 2-1 16 13-1 16.2 11-2 17 17-2 14,16 -
STR鉴定图
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参考文献
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影响因子 :4.9270
引用产品:CL-0190
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Tantalum boride as a biocompatible coating to improve osteogenesis of the bionano interface(2020/03/29)
作者:Ruiyan Li, Guancong Liu, Lina Yang
期刊:J Biomed Mater Res A
影响因子 :4.854
引用产品:CL-0190
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Downregulated fat mass and obesity-associated protein inhibits bone resorption and osteoclastogenesis by nuclear factor-kappa B inactivation(2021/8/29)
作者:Jinpeng Zhuang, Hua Ning, Maoqing Wang
期刊:Cellular Signalling
DOI:10.1016/j.cellsig.2021.110137
影响因子 :4.8500
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O-alkyl and o-benzyl hesperetin derivative-1L attenuates inflammation and protects against alcoholic liver injury via inhibition of BRD2-NF-κB signaling pathway(2021/12/30)
作者:JuanJuan Li, XinYi Lu, Pengcheng Jia
期刊:Toxicology
影响因子 :4.5710
引用产品:CL-0190
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Osteoblast Jmjd3 regulates osteoclastogenesis via EphB4 and RANKL signalling(2022/02/18)
作者:Rui Wang, Huikun Luo, Yang Di
期刊:Oral Diseases
影响因子 :4.0680
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Interleukin-35 from Interleukin-4-Stimulated Macrophages Alleviates Oxygen Glucose Deprivation/Re-oxygenation-Induced Neuronal Cell Death via the Wnt/β-Catenin Signaling Pathway(2022/02/12)
作者:Taoxiao Qu, Hongtao Zeng, Zhiqing Li
期刊:Neurotoxicity Research
DOI:10.1007/s12640-022-00478-7
影响因子 :3.9780
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Synthesis of spiroindolenine-3,3′-pyrrolo[2,1-b]quinazolinones through gold(I)-catalyzed dearomative cyclization of N-alkynyl quinazolinone-tethered indoles(2022/02/08)
作者:Wang Wang, PeiSen Zou, Li Pang
期刊:Organic & Biomolecular Chemistry
影响因子 :3.8900
引用产品:CL-0190
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β-Glucan from Saccharomyces cerevisiae alleviates oxidative stress in LPS-stimulated RAW264.7 cells via Dectin-1/Nrf2/HO-1 signaling pathway(2021/04/21)
作者:Chunwei Yu, Hui Chen, Donghua Du
期刊:Cell Stress & Chaperones
DOI:10.1007/s12192-021-01205-5
影响因子 :3.827
引用产品:CL-0190
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Microstructured titanium functionalized by naringin inserted multilayers for promoting osteogenesis and inhibiting osteoclastogenesis(2021/08/10)
作者:Ke Shen, Xiaojing Zhang, Qiang Tang
期刊:Journal Of Biomaterials Science-Polymer Edition
DOI:10.1080/09205063.2021.1949098
影响因子 :3.682
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Coenzyme Q10 inhibits RANKL-induced osteoclastogenesis by regulation of mitochondrial apoptosis and oxidative stress in RAW264.7 cells(2021/3/23)
作者:Delu Zheng, Chenli Cui, Chen Shao
期刊:Journal Of Biochemical And Molecular Toxicology
影响因子 :3.6420
引用产品:CL-0190
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Silencing of IGF2BP1 restrains ox-LDL-induced lipid accumulation and inflammation by reducing RUNX1 expression and promoting autophagy in macrophages(2022/02/18)
作者:Mingxin Liu, Guizhou Tao, Yiming Cao
期刊:Journal Of Biochemical And Molecular Toxicology
影响因子 :3.5680
引用产品:CL-0190
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Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4-mediated signal transduction pathways in RAW264.7 cells(2021/09/08)
作者:Zhiying Ru, Mingsheng Xu, Gaoxiang Zhu
期刊:Food Science & Nutrition
影响因子 :3.5530
引用产品:CL-0190
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Electrochemical sensing of H2O2 released from living cells based on AuPd alloy-modified PDA nanotubes(2019/2/18)
作者:Guangli He, Fengli Gao, Wei Li
期刊:Analytical Methods
影响因子 :3.5320
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α?rhamnrtin?3?α?rhamnoside exerts anti?inflammatory effects on lipopolysaccharide?stimulated RAW264.7 cells by abrogating NF?κB and activating the Nrf2 signaling pathway(2021/9/15)
作者:Jiangtao Zhou, Kaida Ren, Jing Hou
期刊:Molecular Medicine Reports
影响因子 :3.4230
引用产品:CL-0190
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Placenta?derived mesenchymal stem cells ameliorate lipopolysaccharide?induced inflammation in RAW264.7 cells and acute lung injury in rats(2020/6/15)
作者:Wen Yuan, Hengya Song, Jie Xiong
期刊:Molecular Medicine Reports
影响因子 :3.4230
引用产品:CL-0190
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Folate receptor-targeting mesoporous silica-coated gold nanorod nanoparticles for the synergistic photothermal therapy and chemotherapy of rheumatoid arthritis(2021/1/18)
作者:Xiangyu Li, Yufei Hou, Xiangxue Meng
期刊:RSC Advances
影响因子 :3.3610
引用产品:CL-0190
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Production of a novel Poria cocos immunomodulatory protein in Pichia pastoris: cloning, expression, purification and activities assays(2019/1/24)
作者:Hongbo Li, Xiufen Bu, Kuai Li
期刊:World J Microbiol Biotechnol
影响因子 :3.3120
引用产品:CL-0190
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Niloticin binds to MD-2 to promote anti-inflammatory pathway activation in macrophage cells(2022/10/31)
作者:Guirong Chen, Chang Liu, Mingbo Zhang
期刊:INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY
影响因子 :3.2980
引用产品:CL-0190
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Anti-Inflammatory and Antinociceptive Properties of Flavonoids from the Fruits of Black Mulberry (Morus nigra L.)(2016/4/5)
作者:Hu Chen, Junsong Pu, Dan Liu
期刊:PLoS One
DOI:10.1371/journal.pone.0153080
影响因子 :3.2400
引用产品:CL-0190
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Six undescribed derivatives of stilbene isolated from Lindera reflexa Hemsl. and their anti-tumor and anti-inflammatory activities(2022/10/13)
作者:Yuhang Fu, Yadi Hou, Yizhe Duan
期刊:FITOTERAPIA
DOI:10.1016/j.fitote.2022.105331
影响因子 :3.2040
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Curcumin prevents osteocyte apoptosis by inhibiting M1‐type macrophage polarization in mice model of glucocorticoid‐associated osteonecrosis of the femoral head(2020/02/03)
作者:Shengyang Jin, Chunqing Meng, Yu He
期刊:Journal of Orthopaedic Research
影响因子 :3.102
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Structural characterization and biological activities of a new polysaccharide isolated from Morchella Sextelata(2022/04/13)
作者:Zuo Zhang, Menghua Shi, Hanyu Zheng
期刊:GLYCOCONJUGATE JOURNAL
DOI:10.1007/s10719-022-10058-8
影响因子 :3.0090
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Targeting androgen receptor with ASC-J9 attenuates cardiac injury and dysfunction in experimental autoimmune myocarditis by reducing M1-like macrophage(2017/2/27)
作者:Wenhan Ma, Ya Wang, Shuai Lu
期刊:Biochemical And Biophysical Research Communications
DOI:10.1016/j.bbrc.2017.02.123
影响因子 :2.9850
引用产品:CL-0190
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LncRNA XIST facilitates S1P-mediated osteoclast differentiation via interacting with FUS(2022/01/23)
作者:Dawei Zhang,?Honggang Wang,?Kuibo Zhang?
期刊:Journal Of Bone And Mineral Metabolism
DOI:10.1007/s00774-021-01294-3
影响因子 :2.9760
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Antimicrobial peptide HI-3 from Hermetia illucens alleviates inflammatory of lipopolysaccharide-stimulated RAW264.7 cells via suppression nuclear factor κappa-B signaling pathway(2022/10/13)
作者:Xiaoyan Xu, Hongxia Sun, Jiamin Gao
期刊:MICROBIOLOGY AND IMMUNOLOGY
影响因子 :2.9620
引用产品:CL-0190
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Phytochemical Profile and Anti-Inflammatory Activity of the Fraction from Artemisia lavandulaefolia(2021/2/17)
作者:Laibin Zhang, Limin Guo, Fenglong Wang
期刊:Chemistry & Biodiversity
影响因子 :2.7450
引用产品:CL-0190
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Nitric Oxide Production Inhibitory Eudesmane-Type Sesquiterpenoids from Artemisia argyi(2020/6/23)
作者:Laibin Zhang, Xiaona Nie, Jiajing Chang
期刊:Chemistry & Biodiversity
影响因子 :2.7450
引用产品:CL-0190
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Lignans from Lepidium meyenii and Their Anti-Inflammatory Activities(2021/6/17)
作者:Yi yan Zou, Dai wei Wang, Yong ming Yan
期刊:Chemistry & Biodiversity
影响因子 :2.7450
引用产品:CL-0190
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Commiphoranes K?O, New Terpenoids from Resina Commiphora and Their Anti-Inflammatory Activities(2021/6/2)
作者:Xiaohua Yang, Daiwei Wang, Yongming Yan
期刊:Chemistry & Biodiversity
影响因子 :2.7450
引用产品:CL-0190
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Curcumin prevents osteocyte apoptosis by inhibiting M1‐type macrophage polarization in mice model of glucocorticoid‐associated osteonecrosis of the femoral head(2020/02/03)
作者:Shengyang Jin, Chunqing Meng, Yu He
期刊:Journal of Orthopaedic Surgery and Research
影响因子 :2.6770
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MiR-192-5p suppresses M1 macrophage polarization via epiregulin (EREG) downregulation in gouty arthritis(2021/10/12)
作者:Lixin An, Fuzai Yin
期刊:Tissue & Cell
DOI:10.1016/j.tice.2021.101669
影响因子 :2.5860
引用产品:CL-0190
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Two new lanostane-type triterpenoids from the fungus Periconia sp. TJ403-rc01(2021/11/02)
作者:Weixi Gao, Fengli Li, Shuang Lin
期刊:Natural Product Research
DOI:10.1080/14786419.2021.1998046
影响因子 :2.4880
引用产品:CL-0190
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MiR-382-5p suppresses M1 macrophage polarization and inflammatory response in response to bronchopulmonary dysplasia through targeting CDK8: Involving inhibition of STAT1 pathway(2021/7/22)
作者:Yuanyuan Lv, Yang Li, Jiangya Wang
期刊:Genes to cells
影响因子 :2.3000
引用产品:CL-0190
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Ferric Ion Induction of Triggering Receptor Expressed in Myeloid Cells‐2 Expression and PI3K/Akt Signaling Pathway in Preosteoclast Cells to Promote Osteoclast Differentiation(2020/07/30)
作者:Lulin Liu, Zihou Cao, Chunlei He
期刊:ORTHOPAEDIC SURGERY
DOI:10.1111/os.12750
影响因子 :2.2790
引用产品:CL-0190
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Chemical constituents of the roots of Eupatorium chinense and their anti-inflammatory activities(2022/01/20)
作者:Na Yu, Jinqiang Wang, Xiaoqin Yu
期刊:Phytochemistry Letters
DOI:10.1016/j.phytol.2022.01.006
影响因子 :1.8730
引用产品:CL-0190
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Artemargyinolide E, a new sesquiterpene lactone from Artemisia argyi inhibits inflammatory responses via down-regulating NF-κB signaling pathway(2020/1/22)
作者:Laibin Zhang, Huihui Zhu, Limin Guo
期刊:Phytochemistry Letters
DOI:10.1016/j.phytol.2020.01.009
影响因子 :1.8730
引用产品:CL-0190
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Small Molecule Constituents of Periplaneta americana and Their IL-6 Inhibitory Activities(2021/9/1)
作者:Huasheng Zhang, Yongming Yan, Daiwei Wang
期刊:Natural Product Communications
影响因子 :1.4960
引用产品:CL-0190
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A Novel Phenoxazine-based Fluorescent Probe for the Detection of HOCl in Living Cells(2020/5/5)
作者:Jiajia Yang, Yusi Yao, Yang Shen
期刊:Zeitschrift Fur Anorganische Und Allgemeine Chemie
影响因子 :1.4140
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A New Cycloartanyl Ester from the Roots of Codonopsis pilosula and Its Anti-Inflammatory Activity(2021/1/23)
作者:Jiankuan Li, Junqing Zhang, Lingya Cao
期刊:Chemistry Of Natural Compounds
DOI:10.1007/s10600-021-03295-0
影响因子 :0.8300
引用产品:CL-0190
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The Anti-Inflammatory Effect of 6% HES 200/0.5 on RAW264.7 Cells Induced by LPS through HMGB1/NF-κB Signaling Pathway(2022/10/27)
作者:Jie Zhang, Yongli Wang, Jianzhong Zhang
期刊:Journal of Hard Tissue Biology
影响因子 :0.5930
引用产品:CL-0190
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Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis(2022/09/01)
作者:Xingyan Zhang, Xiaoyan Lian, Huling Li
期刊:Chinese Herbal Medicines
DOI:10.1016/j.chmed.2021.03.002
影响因子 :0.0000
引用产品:CL-0190
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Combination therapy of chitosan, gynostemma, and motherwort alleviates the progression of experimental rat chronic renal failure by inhibiting STAT1 activation(2018/01/10)
作者:Wenxia Bai, Shudong Wang, Shanshan An
期刊:Oncotarget
影响因子 :0.0000
引用产品:CL-0190